ANALISA KADAR KAFEIN PADA KOPI JENIS ROBUSTA DENGAN MENGGUNAKAN SPEKTOFOTOMETRI ULTRAVIOLET

SHOLEHAH, CITRA WAHYU MARATUS (2019) ANALISA KADAR KAFEIN PADA KOPI JENIS ROBUSTA DENGAN MENGGUNAKAN SPEKTOFOTOMETRI ULTRAVIOLET. Undergraduate thesis, Institut Kesehatan Helvetia.

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Abstract

Coffee is one of the drinks favored by the community, besides having a distinctive smell and taste it also contains high levels of caffeine. Caffeine in the appropriate dosage can have a positive effect, but not all products have levels. The purpose of this study was to determine the caffeine content of Robusta 1001, Temanggung, Gayo, Lampung and Wamena coffee in accordance with the Indonesian National Standard 2983; 2014 and to determine differences in caffeine levels in each Robusta coffee sample.
This reasearch type was experimental with a purposive sampling method. The analytical method used qualitative with Parry, and quantitative Ultraviolet Spectrophotometry at a wavelength of 274nm. Validation parameters specified were precision, linearity, LOD, and LOQ.
The results of a qualitative analysis of all positive samples contained caffeine. The average quantitative results of caffeine were Robusta coffee 1001 (3.6%±2%), Temanggung (5.9%±4.8%), Gayo (8.9%±7.5%), Lampung (7,3%±4.8%), and Wamena (6.9%±3.5%). The validation test results obtained linearity r = .9962, LOD and LOQ that was .9478μg/ml and 3.1594μg/ml, RSD value was .56%.
It can be concluded that all samples meet the requirements of the Indonesian National Standard 2983; 2014 with a minimum content of 2.5%. The highest caffeine content is Gayo (8.9%±7.5%) and the lowest is 1001 (3.6%±2%). It is suggested that future researchers obtain more significant levels using other methods such as HPLC.

Keywords: Caffeine, Robusta Coffee, Ultraviolet Spectrophotometry, Indonesian National Standard.

Item Type:	Doktoral (Undergraduate)
Additional Information:	Kata Kunci: Kafein, Kopi Robusta, Spektrofotometri Ultraviolet, Standar Nasional Indonesia.
Subjects:	Fakultas Farmasi dan Kesehatan > Farmasi > Riset Farmasi
Divisions:	Fakultas Farmasi dan Kesehatan > S1 Farmasi
Depositing User:	Citra Wahyu Maratus Sholehah
Date Deposited:	27 Feb 2020 02:55
Last Modified:	27 Feb 2020 02:55
URI:	http://repository.helvetia.ac.id/id/eprint/2347

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